ABSTRACT
Background: Burkholderia cepacia is highly virulent and multidrug resistant organism to cause fatal and serious infections in ICUs leads to rise in mortality and morbidity. aim of present study was to know the prevalence of Burkholderia cepacia in blood stream infection in Intensive Care Unit and to know the drug susceptibility.Methods: This is a prospective study was carried out in the Intensive Care Unit and Department of Microbiology, Narayana Medical College, Nellore, from February to March 2018. As a part of routine investigations Blood, urine, sputum or tracheal secretions sent for culture and sensitivity to the Microbiology laboratory. By conventional method, all the samples were cultured (except blood) onto Blood agar, Chocolate agar and MacConkey, s agar; incubated for 18-24 hours at 37?C. Blood cultures were performed in BACT/ Alert 3D (Biomeriux), only positives were subculture by conventional method. Further analysis was done in culture positive samples only.Results: A total of 448 patients admitted in ICU were included in the study, from them 586 samples were collected. out of which we got 238 culture positives. Among them 19 patients were positive for Burkholderia cepacia, most of them isolated from blood (78.9%), followed by respiratory secretions (21.1%) and none of them were isolated from urine samples. Most of the isolates were sensitive to Meropenam and Tigecycline (89.4%) followed by minocycline (84.2%), ceftazidime (73.6%), levofloxacin (63.1%). While B. cepacia isolates showed high resistance to cefaperazone-sulbactam, ciprofloxacin, ticarcillin-clavulanic acid with (84.2%), (89.4%), (89.4%) respectively.Conclusions: To conclude that, Burkholderia cepacia is one of the emerging causes of septicemia with multidrug resistance, cross contamination may be the root cause so it should be treated quickly and effectively.
ABSTRACT
Type-2 diabetes women are more prone for Urinary tract infections (UTI). Many UTIs are asymptomatic and whether the symptomatic UTI are preceded by asymptomatic bacteriuria (ASB) is not clear. Hence, the present study is conducted to understand the relationship between ASB and symptomatic UTI among Type-2 diabetic women along the sea coast.A hospital based study conducted in the Narayana Medical College & Hospital, which is situated within 10-15 km radius of the seacoast in Nellore district of Andhra Pradesh, India. 238 subjects were selected, out of these 164 diabetic subjects in the experimental and 56 in the control group. Findings indicate that the prevalence of ASB in type-2 diabetic women is high along the sea coast and significantly associated with nephropathy. The major risk factors include age, duration of diabetes, proteinuria, leucocyturia, glucosuria, nephropathy and rise in plasma glucose. Among the clinical isolates of ASB, gram negative isolates were more than gram positive. These isolates are highly resistant to antibiotics like Ciprofloxacin, Ofloxacin, Gentamicin and Cefotaxime while, sensitive to Amikacin and Cephaperazone ± sulbactam. Thus present study concludes that overall risk factors for ASB in type-2 diabetic women observed are proteinuria, leucocyturia, glucosuria, nephropathy and plasma glucose.